| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Bordetella bronchiseptica | TTGCGTGATGATCCTGCCC | TGCGACGCACGATTTCCA | 60.45 | 60.36 | 284 | 95 |
100.00%
|
100.00%
|
| Primer 2 | Bordetella bronchiseptica | TCGACGTGCTGGTCAACA | ACGTTGATGTGCTTGCGC | 59.19 | 59.75 | 289 | 92 |
100.00%
|
100.00%
|
| Primer 3 | Bordetella bronchiseptica | ACGCAGGTTCAAGACGCT | AGCAGGCCTTCGGAAATCC | 59.58 | 60.08 | 177 | 88 |
100.00%
|
100.00%
|
| Primer 4 | Bordetella bronchiseptica | ACGCAGGTTCAAGACGCT | TCAGCAGGCCTTCGGAAA | 59.58 | 58.84 | 179 | 88 |
100.00%
|
100.00%
|
| Primer 5 | Bordetella bronchiseptica | ACGCAGGTTCAAGACGCT | GTCAGCAGGCCTTCGGAAA | 59.58 | 60.30 | 180 | 88 |
100.00%
|
100.00%
|
| Primer 6 | Bordetella bronchiseptica | AAAAGCTGTCGTGCGTGC | TCAATGGGTGGCGAAGTGG | 59.67 | 60.30 | 200 | 88 |
100.00%
|
100.00%
|
| Primer 7 | Bordetella bronchiseptica | TCGTCAACCGCAGTCCAT | AACGCTTGCCGAAGTGGA | 58.94 | 59.89 | 291 | 87 |
100.00%
|
100.00%
|
| Primer 8 | Bordetella bronchiseptica | GACCCATCAACCTGCACCA | ACGTTGATGTGCTTGCGC | 59.93 | 59.75 | 190 | 85 |
100.00%
|
100.00%
|
| Primer 9 | Bordetella bronchiseptica | TCGACGTGCTGGTCAACAA | ACGTTGATGTGCTTGCGC | 59.86 | 59.75 | 289 | 85 |
100.00%
|
100.00%
|
| Primer 10 | Bordetella bronchiseptica | ACCCATCAACCTGCACCAC | ACGTTGATGTGCTTGCGC | 60.23 | 59.75 | 189 | 85 |
100.00%
|
100.00%
|